Microscopy:Article Title: Self-assembled 3D RNA cage nanoparticles
Article Snippet: Then the girds were transferred to JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with incolumn filter for screening. .. Micrographs of 10 nm RNA prism were recorded with a direct detection device (DDD) (DE-20 4 k×5 k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000× microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ̃20 electrons per second per Å2 with a total exposure time of 3 s. Micrographs of 5 nm RNA prism were recorded with a 4 k×4 k CCD (Gatan) at 80,000× microscope magnification (corresponding to a calibrated sampling of 1.36 Å per pixel) and a dose rate of ̃20 electrons per second per Å2 with a total exposure time of 3 s. A total 52 images of 10 nm RNA prism and 30 images of 5 nm RNA prism were collected with a defocus range of 2-4 μM. .. Single Particle Image Processing and 3D Reconstruction The image processing software package EMAN2 was used for the micrograph evaluation, particle picking, CTF correction, 2-D reference-free class averaging, initial model building and 3-D refinement of the cryo-electron microscopy data.
Article Title: Fabrication of RNA 3D Nanoprism for Loading and Protection of Small RNAs and Model Drugs
Article Snippet: Then the girds were transferred to JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with in-column filter for screening. .. Micrographs of 10 nm RNA prism were recorded with a direct detection device (DDD) (DE-20 4k×5k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000×microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ~20 electrons per second per Å 2 with a total exposure time of 3 s. Micrographs of 5 nm RNA prism were recorded with a 4k × 4k CCD (Gatan) at 80,000× microscope magnification (corresponding to a calibrated sampling of 1.36 Å per pixel) and a dose rate of ~20 electrons per second per Å 2 with a total exposure time of 3 s. A total 52 images of 10 nm RNA prism and 30 images of 5 nm RNA prism were collected with a defocus range of 2~4 μm. .. The image processing software package EMAN2 was used for the micrograph evaluation, particle picking, CTF correction, 2-D reference-free class averaging, initial model building and 3-D refinement of the cryo-electron microscopy data.
Article Title: Designer nanoscale DNA assemblies programmed from the top down
Article Snippet: All grids were screened on a JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with in-column energy filter with a slit of 20 eV. .. Micrographs of the icosahedron, tetrahedron, cuboctahedron, and octahedron were recorded with a direct detection device (DDD) (DE-20 4k×5k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000× microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ∼21 electrons/sec/Å 2 with a total exposure time of 3 sec. Micrographs of the reinforced cube and the nested cube were recorded on a 4k×4k CCD camera (Gatan, Inc.) at 40,000× microscope magnification (corresponding to a calibrated sampling of 2.95 Å per pixel) and a dose rate of ∼20 electrons/sec/Å 2 with a total exposure time of 3 sec. A total of 180 images for the icosahedron, 91 images for the tetrahedron, 101 images for the cuboctahedron, 100 images for the octahedron, 36 images for the reinforced cube, and 152 images for the nested cube were collected with a defocus range of ∼1.5–4 μm. ..
Sampling:Article Title: Self-assembled 3D RNA cage nanoparticles
Article Snippet: Then the girds were transferred to JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with incolumn filter for screening. .. Micrographs of 10 nm RNA prism were recorded with a direct detection device (DDD) (DE-20 4 k×5 k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000× microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ̃20 electrons per second per Å2 with a total exposure time of 3 s. Micrographs of 5 nm RNA prism were recorded with a 4 k×4 k CCD (Gatan) at 80,000× microscope magnification (corresponding to a calibrated sampling of 1.36 Å per pixel) and a dose rate of ̃20 electrons per second per Å2 with a total exposure time of 3 s. A total 52 images of 10 nm RNA prism and 30 images of 5 nm RNA prism were collected with a defocus range of 2-4 μM. .. Single Particle Image Processing and 3D Reconstruction The image processing software package EMAN2 was used for the micrograph evaluation, particle picking, CTF correction, 2-D reference-free class averaging, initial model building and 3-D refinement of the cryo-electron microscopy data.
Article Title: Fabrication of RNA 3D Nanoprism for Loading and Protection of Small RNAs and Model Drugs
Article Snippet: Then the girds were transferred to JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with in-column filter for screening. .. Micrographs of 10 nm RNA prism were recorded with a direct detection device (DDD) (DE-20 4k×5k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000×microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ~20 electrons per second per Å 2 with a total exposure time of 3 s. Micrographs of 5 nm RNA prism were recorded with a 4k × 4k CCD (Gatan) at 80,000× microscope magnification (corresponding to a calibrated sampling of 1.36 Å per pixel) and a dose rate of ~20 electrons per second per Å 2 with a total exposure time of 3 s. A total 52 images of 10 nm RNA prism and 30 images of 5 nm RNA prism were collected with a defocus range of 2~4 μm. .. The image processing software package EMAN2 was used for the micrograph evaluation, particle picking, CTF correction, 2-D reference-free class averaging, initial model building and 3-D refinement of the cryo-electron microscopy data.
Article Title: Designer nanoscale DNA assemblies programmed from the top down
Article Snippet: All grids were screened on a JEM2200FS cryo-electron microscope (JEOL) operated at 200 kV with in-column energy filter with a slit of 20 eV. .. Micrographs of the icosahedron, tetrahedron, cuboctahedron, and octahedron were recorded with a direct detection device (DDD) (DE-20 4k×5k camera, Direct Electron, LP) operating in movie mode at a recording rate of 25 raw frames per second at 25,000× microscope magnification (corresponding to a calibrated sampling of 2.51 Å per pixel) and a dose rate of ∼21 electrons/sec/Å 2 with a total exposure time of 3 sec. Micrographs of the reinforced cube and the nested cube were recorded on a 4k×4k CCD camera (Gatan, Inc.) at 40,000× microscope magnification (corresponding to a calibrated sampling of 2.95 Å per pixel) and a dose rate of ∼20 electrons/sec/Å 2 with a total exposure time of 3 sec. A total of 180 images for the icosahedron, 91 images for the tetrahedron, 101 images for the cuboctahedron, 100 images for the octahedron, 36 images for the reinforced cube, and 152 images for the nested cube were collected with a defocus range of ∼1.5–4 μm. ..
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